In this manuscript we show that the RPG protein is a central polarizing protein and organizes the so-called “infectosome” at the tip of progressing infection threads and in the nuclear periphery.
Although technically challenging, the application of FLIM imaging allowed us to discriminate fluorophores from autofluorescence in a scientifically sound way and to image infectosomes with highest precision.
Lace B, Su C, Invernot-Perez D, Rodriguez-Franco M, Vernié T, Batzenschlager M, Egli S, Liu CW, Ott T (2022)
RPG acts as a central determinant for infectosome formation and cellular polarization during intracellular rhizobial infections
pre-printed deposited at: https://www.biorxiv.org/content/10.1101/2022.06.03.494689v2 (open access)