We are running a Philps CM-10 (100kV) Transmission Electron Microscope (TEM), which can be used in frame of collaborations and service applications. The system and service is maintained by a full-time staff scientist and half-time technician.
We are offering full sample processing and analyses including preparation, sectioning, staining and imaging. Sample types that have recently been processed include but are not limited to: plants (leaves, shoots, roots, fruits), sections from animal tissues, cell cultures, bacteria, archeae, etc.
For sample preparation and analysis the following methods are available:
- Negative staining
- Chemical fixation of cells and multi-cellular specimens
- Cryo-fixation (High Pressure Freezing) and Freeze Substitution
- Resin embedding
- Ultramicrotomy (including serial sectioning)
- Cryo-ultramicrotomy (for Tokuyasu technique)
Furthermore we offer and/or are involved in:
- Advanced training of the users on all the accessible techniques.
- Participating in courses and lectures on EM methods in cell biology.
In addition we are continuously aiming to broaden our methods portfolio. Thus, we are currently developing
- a Correlative Light Electron Microscopy (CLEM) approach with the aim to trace symbiotic and pathogenic infections of single plant cells (one out of thousands) within a multi-cellular tissue context.
- a Correlative Nanoscale Membrane Imaging Platform (co-NAMIP) that should provide a robust workflow allowing the coordinated analysis of membrane nanodomains using advanced fluorescence as well as electron microscopy.