Correlative Light Electron Microscopy (CLEM) offers the fascinating opportunity to initially observe structures and/or protein complexes by advanced fluorescence microscopy techniques and precisely track back these individual sites in an electron microscopical setup. While CLEM has been frequently used to study rather ubiquitous cellular processes (so those occurring continuously in the cell type of interest) we currently aim to establish a robust workflow that allows the initial tracing of comparably rare events (such as infections of individual host cells within a multi-cellular complex tissue) by fluorescence microscopy and their subsequent analysis at the highest possible resolution using transmission electron microscopy (TEM).
To establish a reliable and efficient workflow, we are currently setting up a novel Nanoscale Membrane Imaging Platform (NaMIP). Read more.